Pig heart fumarase really does exhibit negative kinetic co-operativity at a constant ionic strength.
نویسندگان
چکیده
The kinetics of the action of fumarase on L-malate and fumarate were investigated at constant ionic strength. This was done to evaluate reports that fumarase follows simple Michaelis-Menten kinetics. However, when pH, buffer concentration and ionic strength are all maintained at constant values, the Lineweaver-Burk plots exhibit pronounced downward curvature, characteristic of negative kinetic co-operativity.
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ورودعنوان ژورنال:
- The Biochemical journal
دوره 235 3 شماره
صفحات -
تاریخ انتشار 1986